Maximum chance phylogenetic relationships of control area...

Maximum chance phylogenetic relationships of control area…

Optimum chance phylogenetic relationships of control area haplotypes based on market purchased eyeball examples and sequences acquired from understood people.

Phylogenetic relationships had been identical in Bayesian inference, maximum likelihood and maximum parsimony estimates (?lnL = 1399.28612; parsimony size = 141). Help values mentioned above nodes are Bayesian probabilities according to 5,000,000 resamples, maximum chance bootstrap help values predicated on 1,000 bootstrap replicates, and maximum parsimony bootstrap help values according to 2,000 bootstrap replicates. Support values are shown limited to clades of Sotalia guianensis and Sotalia fluviatilis haplotypes, and for the monophyly of Sotalia. Branch lengths are proportional to likelihood that is maximum divergence; scale shows portion of series divergence. Haplotypes 1–14, highlighted in bold, were present in our correspond and study to GenBank accession #EU022531–EU022544.

To help expand confirm the relationships for the unknown sequences, we performed a nonphylogenetic analysis that is cladistic. We constructed a matrix of autapomorphies for Sotalia fluviatilis, Sotalia guianensis, Inia g. Geoffrensis, Inia g. Humboltiana, and Inia g. Boliviensis predicated on available series information in Genbank and our guide people, and performed a populace aggregation analysis ( Davis and Nixon 1992 ).

All eyeball?derived sequences decided by BLAST to be of cetacean origin provided species?specific autapomorphic character states indicative of molecular positional homologies with Sotalia guianensis with no other types, unambiguously assigning all eyeballs of cetacean origin to Sotalia guianensis. The control area and Cytochrome b information sets have two and six diagnostic internet web internet sites, correspondingly, that assign all cetacean eyeball examples to Sotalia guianensis and unambiguously differentiate it from Sotalia fluviatilis and Inia spp. ( dining dining Table 1; online Appendix S1, S2).

bbw cams

Control area BEL MAO PVH
Variable position 39 136 139 160 175 231 256 269 270 362 381 387 404 463 504 559 Haplotype occurence
Inia spp. A T the G C G G/A A A T C C/T C/T C C/T A ? ? ?
Sotalia fluviatilis T T A G C/T G C/T T C C/T C T T C T A ? ? ?
Sotalia guianensis C G/T A/C A C/T G/A C/T C/T C/T C/T C/G C/T C/T C/T C/T C/A 22 11 1
CR_Hap01 C T A A T A C C C C C T C C T the 1 ? ?
CR_Hap02 C T A A T A C T C C C C T C T A 2 ? ?
CR_Hap03 C T A A T A C C C C C C T C T the 6 2 ?
CR_Hap04 C T A A T A C T C C C T C C T A 3 5 1
CR_Hap05 C T A A T A C T C T C T C C T A 1 2 ?
CR_Hap06 C T A A C the T T T C C T T C T A 1 ? ?
CR_Hap07 C T A A T A C T C C C C C C T A 3 ? ?
CR_Hap08 C G C A T G C T C C C T C C T the 1 ? ?
CR_Hap09 C T A A T G C T C C C T C C T the 1 ? ?
CR_Hap10 C T A A T A C C C C C T T C T A 1 ? ?
CR_Hap11 C T A A T A C C C C C C T T T A ? 1 ?
CR_Hap12 C T A A T A C C C C C C T C T C ? 1 ?
CR_Hap13 C T the A C the C T T C C T T C C the 1 ? ?
CR_Hap14 C T A A T A C C C C G C T C T the 1 ? ?
Cytochrome b
adjustable position 49 78 124 297 367 396 462 501 528 531 561
Inia spp. G T A C G C T T A C T ? ? ?
Sotalia fluviatilis G C A G G C T the A C T ? ? ?
Sotalia guianensis G/A C/T G/A A G/A C/T C G C T C 16 7 1
CB_Hap01 G C A A G C C G C T C 8 4 1
CB_Hap02 A C A the G C C G C T C 1 ? ?
CB_Hap03 G T A A G C C G C T C 1 ? ?
CB_Hap04 G C G A G T C G C T C 3 1 ?
CB_Hap05 G C G A A T C G C T C 3 2 ?
  • Web web web Sites highlighted in bold unambiguously assign the unknown eyeball samples to Sotalia guianensis. Those perhaps perhaps not highlighted differentiate haplotypes that are observed. Control area haplotypes 1–14 match GenBank accession numbers EU022531?EU022544, while cytochrome b haplotypes 1–5 match GenBank accession numbers EU022545?EU022549. BEL = Belem, MAO = Manaus, PVH = Porto Velho.

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